Structural determination and characterization of a 40 kDa protein isolated from rat 40 S ribosomal subunit

Release of the nonribosomal proteins from the mammalian native 40 S ribosomal subunit by aurintricarboxylic acid.

Purification and characterization of a 40 S ribosomal protein S6 kinase from vanadate-stimulated Swiss 3T3 cells.

Recently we have identified a mitogen-activated S6 kinase from Swiss 3T3 cells (Jenö, P., Ballou, L. M., Novak-Hofer, I., and Thomas, G. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 406-410). Here we describe the detailed purification of this enzyme from high-speed supernatants (400,000 x g) of vanadate-treated cell extracts. The enzyme is purified through six sequential steps including cation- and...

Isolation of a 40-kDa Huntingtin-associated protein.

Huntington's disease is caused by an expanded CAG trinucleotide repeat coding for a polyglutamine stretch within the huntingtin protein. Currently, the function of normal huntingtin and the mechanism by which expanded huntingtin causes selective neurotoxicity remain unknown. Clues may come from the identification of huntingtin-associated proteins (HAPs). Here, we show that huntingtin copurifies...

40-De­oxy-40(S)-iodo­rapamycin

The title compound, C(51)H(78)INO(12), contains a 29-membered ring incorporating amide, lactone and ester groups. It contains a total of 15 stereogenic centres. In the crystal, mol-ecules are linked by O-H⋯O hydrogen bonds, forming C(8) chains propagating in [100]. A weak intra-molecular O-H⋯O inter-action also occurs.

Arabidopsis p40 homologue. A novel acidic protein associated with the 40 S subunit of ribosomes.

We have isolated a full-length cDNA clone from Arabidopsis thaliana that has extensive homology to p40 proteins from other organisms. The cDNA predicts a protein (A-p40) of 298 amino acids with a calculated molecular mass of 32.5 kDa and a pI of 4.8. Antibodies raised against an A-p40 fusion protein detected a polypeptide of about 40 kDa in Arabidopsis and about 42 kDa in corn and soybean. In t...